The most advanced portable modulated chlorophyll fluorometer available, with FM' correction option, according to Loriaux 2013, included for all light adapted protocols including quenching.
The OS5p+ portable system - includes the latest advancements in variable chlorophyll fluorescence measurements. The OS5p+ can measure most types of plant stress, and offers a wider range of up to date measuring protocols and automation.
Wide Range of Programmable Testing Protocols including:
The OS5p+ is designed to be the most advanced chlorophyll fluorometer available. It is intended for advanced plant research and plant stress measurement. It offers the latest updates in chlorophyll fluorescence capabilities, including: FM' correction according to Loriaux 2013, a stable white actinic white light source that allows chloroplast migration, and quenching relaxation measurement of qE, qT, qM, qZ, and qI. It also provides a comprehensive list of quenching protocols including Kramer lake model, Hendrickson lake model, and puddle model protocols. Other capabilities include: A Y(II) protocol, an FV/FM protocol, Strasser OJIP, Vredenberg OJIP, and Rapid Light Curves (for changing light conditions).
Parameters Measured and Protocols included:
Quantum Photosynthetic Yield of PSII (or F/FM' or Y).
Electron transport rate (w/optional clip).
Photosynthetically Active Region value (with optional PAR clip).
Leaf temperature (with optional PAR clip).
Maximum Photochemical efficiency of PSII.
A more sensitive detector of stress than FV/FM, but it does not measure plant efficiency.
FMS (or FM'):
Maximal fluorescence with actinic illumination at steady state fluorescence.
FS (or F):
Fluorescence under steady state conditions (prior to saturation pulse).
A measure of a leaf's photosynthetic capacity or maximum electron transport rate a is the initial slope of line at low PAR values created by relating ETR to PAR. It provides a measure of quantum efficiency.
A measurement of the light intensity where light saturation dominates, or the minimum saturation level.
Hendrickson Quenching with NPQ (standard):
Y(NPQ), Y(NO), Y(II), NPQ, FV/FM.
Kramer Quenching (standard):
qL, Y(NPQ), Y(NO), Y(II), FV/FM.
Puddle model parameters (standard):
NPQ, qN, qP, Y(II), FV/FM.
Quenching relaxation protocol (standard):
qE, qM, qZ, qT, qI along with either puddle model, or Hendrickson parameters. Adjustable times for each.
OJIP - Vredenberg OJIP quenching protocol:
OJIP - Strasser Protocol:
Direct readout of: OJIP t100μs, t300μs (or K step), tFM (or time to P or FM), A (or area above the curve), MO (or RC/ABS), PIABS (or performance index).
Strasser OJIP parameters reported to the data file only:
ABS/RC, TRO/RC, DIO/CS, ETO/RC, TRO/ABS, ETO/TRO, ETO/CS,RC/CSO, RC/CSM , S, M, T.
White LED with 690 nm short pass filter. 15,000 μmols, 7,500 μmols with PAR clip.
Optional red saturation light source.
Red: 660 nm LED with 690 nm short pass filter.
Blue: 450 nm LED.
Actinic light source:
White LED to 5,800 μmols for OJIP, and to 1,850 μmols in PAR Clip.
Optional red actinic light source for OJIP.
Far red light:
Above 740 nm.
Pulse modulation method.
Detector & Filters:
A PIN photodiode with a 700 ~ 750 nm bandpass filter.
Auto-switching from 1 to 10,000 points per second, depending on test & on phase of test.
Automated routine to optimally set the modulated light intensity. The modulated light may also be set manually.
Multi-Flash FM' correction for all light adapted protocols & quenching. It may be turned on or off.
Adjustable from .1 seconds to 12 hours.
1 Gigabyte of non-volitile flash memory, supporting unlimited data sets and traces.
USB, SD/MMC 1 Gigabyte data cards.
Graphic color touch screen.
Menu driven touch screen.
Internal 12V rechargeable nickel metal hydride battery.
8 to 12 hours of continuous operation.
7 in x 5.5 in x 3.25 in.
17.8 cm x 14 cm 8.3 cm.
0 to 50oC.
Up to 32 traces can be overlaid on the graphic display screen. The data file is designed for easy overlay graphing and spider plot graphing. Thousands of measurement parameter sets can be stored in a single data file for spider graphing. The number of data files are only limited by machine memory limits. FO is measured not estimated.
Measuring Y(II) or Δ F/FM' protocol
Measuring Y(II) protocol with Loriaux 2013 FM' correction
Measuring Y(II) with a square topped flash
How FM' correction works
How FM' correction works
Measuring the xanthophyll cycle, chloroplast migration, and photoinhibition using Kramer quenching protocol equations
Measuring the xanthophyll cycle, chloroplast migration, and photoinhibition using Hendrickson quenching protocol equations
Programmable Vredenberg OJIP Investigation
Rapid light curves
Rapid light curve graphs
Protective trabsport case included
Opti-Sciences, Inc. 8 Winn Avenue Hudson NH 03051 USA (603)883-4400